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  • Case Name: Ozone in Grain Storage
  • Case Number: QJ-8005K

Ozone can effectively kill injurious insects,mould in grain depot,improve grain quality.

It is a kind of environmental fumigation agent widely used in different sizes of granary.

With long time use of phosphine,the injurious insects’Drug resistance is getting stronger.

A replacement for chemical fumigants is imperative because insects not only eat the grain,

they defecate on it causing development of fungi, primarily Fusarium and

Aspergillus.

These fungi can release potentially deadly mycotoxins that can cause illness in most livestock

and have been linked to some forms of human cancer.

In reent years,many scientific and technical workers and entomologist have paid a lot

attention to research ozone technology and found that ozone can kill injurious insects,

mould without second pollution since ozone half-time is very short and ozone can become

oxygen after finished working.

2.Effectively kills Worm eggs of injurious insect

Practice has proved that under normal temperature,ozone

concentration 30ml/m3,it can kill 100% maize weevil in 3 d,grain

moth in 6d,tribolium castaneum in 7d and Cryptolestes ferrugineus

in 7d.With the condition above,all the Worm eggs and mould can be

100% killed.

Ozone not only effectively kills injurious insects,mould,but also

useful for reducing grain moisture content.Ozone is not stable in dry

air,so can resolves into oxygen without residue,second pollution on

the surface of grain or inside the barn,very environmental.

3.kills microorganism in grain,sterilization,reduce toxin

during grain mildew and mildew proof

4.useful for longterm grain storage

Application of ozone for reduction of mycological infection in

wheat grain

For this purpose, grains with a moisture content of 23.2 % of the

"Tauras" variety were selected and ventilated daily for 8 hours until

grain wetness was reduced to 14.0 %. The effect of ventilation

duration and ozone impact was evaluated according to the changes

in grain contamination with micromycetes propagules (cfu x g (-1)),

and alternation of micromycetes species on the grain surface. At

drying grains by active ventilation with an ozone--air mixture, at O

(3) concentration of 700 ppb, the drying period was reduced by

about 20 %, and mycological contamination depends on initial grain

moisture content (w): when w=15.2 %, contamination was reduced

by up to 2.2 times, and when w=22.0 %--up to 3 times. At the

same time, the composition of micromycetes species on the grain

surface changed significantly: in non-ventilated grain there were

detected micromycetes of 26 species, and in ventilated grain--of 11

species. Efficient ozone impact was established only when the

mound of wet (w>18.0 %) grains was exposed to ozone.

INHIBITION EFFECT OF OZONE ON TWO KINDS OF MAIN FUNGI IN STORED GRAIN

The inhibition effect of different concentration of ozone and exposure time on Aspergillus

flavus and Aspergillus niger,the main fungi in stored grain,was investigated at room

temperature by measuring the growth of colony,while the effect of ozone fumigation

on their colonial morphology and mycelial structure was also

tested.The results showed that there were obvious inhibition effect

of ozone fumigation on both Aspergillus flavus and Aspergillus

niger,and the inhibition effect became better with ozone

concentration increasing and exposure time extending.Ozone

fumigation could fade the colors of the two kinds of fungi,make

mycelium bend,crack and dilate partly,even lead to cell

lysis.According to the test,it would have better effect against these

two kinds of fungi at 170 mg/m3~190 mg/m3 ozone concentration

for 6 hours exposure.